Enhancement of radio sensitization by gold-silica shell- core nanoparticle in MCF7 breast cancer cells

Authors

  • Abolfazl Akbarzad Tuberculosis and Lung Disease Research Center of Tabriz, Tabriz University of Medical Sciences Department of Chemical Engineering, Northeastern University, Boston, MA, USA
  • Alireza Farajollahi Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Department of Medical Physic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Imam Reza University Hospital, Radiotherapy Department, Tabriz University of Medical sciences, Tabriz, Iran
  • Alireza Naseri Faculty of Advanced Medical Sciences, Tabriz University of Medical Science, Tabriz, Iran
  • Behnam Nasiri motlagh Tabriz Valiasr Hospital, Department of Radiation Oncology, Tabriz, Iran
  • Effat Alizadeh Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran
  • Ghazal Darfarin Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Roya Salehi Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Science, Tabriz, Iran
Abstract:

  Introduction: In the present study, we investigated the role of gold-silica shell-core nanoparticle in megavoltage irradiation of MCF7 breast cancer cells.   Materials and Methods: gold-silicon oxide shell-core nanoparticles (NPs) were obtained by conjugation of gold nanoparticle with amine or thiol functionalized silica nanoparticles (AuN@SiO2 and AuS@SiO2). The structure of each nanoparticle was fully characterized by FTIR and TEM. The NPs treated cells were irradiated by using 6 and 18 MV x-rays. MTT and Brdu assay, DAPI staining and cell cycle were used to evaluate efficiency of nanoparticle in radiation therapy. Results: The average size of AuN@SiO2 and AuS@SiO2 NPs was 23.79±7.04 and 31.28±7.37 nm, respectively. The Brdu findings revealed significantly more death in AuN@SiO2 (amine) treated and exposed cells compared to AuS@SiO2 (thiol) treated cells. (p<0.05). Cell cycle study show that presence of NPs enhanced the effect of radiotherapy, in particular, highest destructive effects were observed at the radiation dose of 8 Gy using energy 18 MV.   Conclusion: It was concluded that our findings may open the possibilities for cooperative radiotherapy and gold-silica shell-core nanoparticle by increasing cytotoxicity in MCF7 cells and decreased side effect from single modality radiotherapy.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Co-enhancement of fluorescence and singlet oxygen generation by silica-coated gold nanorods core-shell nanoparticle

Metal-enhanced fluorescence (MEF) as a newly recognized technology has been attracting considerable attention and is widely used in fluorescence-based technology. In this paper, we reported a novel distance-dependent MEF and metal-enhanced singlet oxygen generation phenomenon based on silica-coated gold nanorods (AuNRs@SiO2) core-shell structure with tetra-substituted carboxyl aluminum phthaloc...

full text

Enhancement of radio-sensitivity of colorectal cancer cells by gold nanoparticles at 18 MV energy

Objective(s): Taking advantage of high atomic number of gold nanoparticles (GNPs) in radiation dose absorbing, many in vitro and in vivo studies have been carried out on using them as radio-sensitizer. In spite of noticeable dose enhancement by GNPs at keV energies, using this energy range for radiotherapy of deep-seated tumors is outdated. The aim of the present work was to examine the effect ...

full text

Evaluation of gold nanoparticles radio sensitization effect in radiation therapy of cancer: review article

In recent years, the use of gold nanoparticles (GNPs) in radiation therapy has been studied by experimentation and Monte Carlo simulation repeatedly. Although the idea of increasing doses has been raised by high-atomic elements since decades ago, but due to the adaptation of gold nanoparticles with the biological system, scientists have incited more about the various uses of these materials in ...

full text

Biomimetic approach to the formation of gold nanoparticle/silica core/shell structures and subsequent bioconjugation.

The encapsulation of individual nanoparticles has gained great attention as a method for both stabilizing nanoparticles and tailoring their surface properties. In particular, the encapsulation of nanoparticles with silica shells is advantageous for bioconjugation and applications to (nano)biotechnology. Herein we report a method for constructing gold nanoparticle (AuNP)/silica core/shell hybrid...

full text

A systematic growth of Gold nanoseeds on Silica for Silica@Gold core-shell nanoparticles and investigation of optical properties

Metal nanoshells consists of a dielectric core surrounded by a thin noble metal shell, possess unique optical properties, which render nanoshells attractive for use in different technologies. This paper reports a facile method for growth of small gold nanoparticles on the functionalized surface of larger silica nanoparticles. Mono-dispersed silica particles and gold nanoparticles were prepared ...

full text

Gradual Growth of Gold Nanoseeds on Silica for Silica@Gold Core-Shell Nanoparticles and Investigation of Optical Properties

Metal nanoshells consists of a dielectric core surrounded by a thin noble metal shell, possess unique optical properties that render nanoshells attractive for use in different technologies. This paper reports a facile method for growth of small gold nanoparticles on the functionalized surface of larger silica nanoparticles. Mono-dispersed silica particles and gold nanoparticles were prepared by...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 15  issue Special Issue-12th. Iranian Congress of Medical Physics

pages  420- 420

publication date 2018-12-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023